                         SEQUENCE LISTING

<110>  Council of Scientific and Industrial Research
 
<120>  DIAGNOSTIC ASSAYS FOR THE DETECTION AND IDENTIFICATION OF 
       ASPERGILLI

<130>  PCT1139

<150>  0375/DEL/2010
<151>  2010-02-19

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  Primer, Y=CT

<400>  1
atctggagaa atgaytgcga tgcygcyat                                         29


<210>  2
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer,  K=GT, Y=CT

<400>  2
tckagaccgg yatggttytc                                                   20


<210>  3
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Probe, Y=CT, K=GT, B=CGT

<400>  3
aaccaygccg gtctkgaycg bggcca                                            26


<210>  4
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  4
taagatgaag caaacatccg aggagt                                            26


<210>  5
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  5
gcgggtggag agacctcagc t                                                 21


<210>  6
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Probe

<400>  6
gtgcaacctc tgatcctacg ttggc                                             25


<210>  7
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  7
cgattgatca caagttggct cgaac                                             25


<210>  8
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  8
tacatgttgc cagattcctc atattcccta g                                      31


<210>  9
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Probe

<400>  9
gcgccaaatg gtccagaagt atgtc                                             25


<210>  10
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  10
caacgcaaaa tatggctact atctcgatca                                        30


<210>  11
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  11
cattgatttc ttccagggtg attccg                                            26


<210>  12
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Probe

<400>  12
gaagcttctt ccacatctca ggcaagga                                          28


<210>  13
<211>  560
<212>  DNA
<213>  Aspergillus

<400>  13
gcttgaggtc tcctgtaagg aagcccatca taatttcttg attgaaagcg atttggtcca       60

gttgattaac cattgtgcct tgtctagacg gtaagatgaa gcaaacatcc gaggagtatg      120

ttcccgggga agctacagag gaggtaactg cagaagcaag tgcaacctct gatcctacgt      180

tggcgaatgc ggcctacact gaactccagg acggccctct tgacgccggt gctacagcag      240

ccaacgagat tgactcctct gcgcctaaag ctgaggtctc tccacccgcg cagactctcg      300

tttccgatgc tgctaatcct gtagccgagg cgtcgtggga acagaacggg gctggctcgc      360

tcgagtcgtc ggcaaatgct gacggctggg ttgaggtgcc tcgcgacccg gcggagacgg      420

agacgggcct acaggccacc cctgcctctg tcgataccgg tctgaaggac aaccagactg      480

tcgctgcggc ctccggacag agtgatgaac atgtctccgt gcctaaggcg cagggcagcg      540

acggattcga acccgttgtg                                                  560


<210>  14
<211>  180
<212>  PRT
<213>  Aspergillus flavus

<400>  14

Thr Leu Leu Glu Gln Val Arg Leu Asp Leu Val Glu Thr Gly Leu Pro 
1               5                   10                  15      


Arg Leu Leu Gln Ser Arg Gln Val Lys Ser Val Thr Ile Val Pro Phe 
            20                  25                  30          


Leu Thr Arg Met Asn Glu Thr Met Ser Asn Ile Leu Pro Val Ser Phe 
        35                  40                  45              


Ile Ser Thr Glu Thr Arg Thr Asp Thr Gly Arg Ala Ile Pro Ala Ser 
    50                  55                  60                  


Gly Arg Pro Gly Ala Gly Lys Cys Lys Leu Ala Ile Val Ser Met Ser 
65                  70                  75                  80  


Gly Arg Phe Pro Glu Ser Pro Thr Thr Glu Ser Phe Trp Asp Leu Leu 
                85                  90                  95      


Tyr Lys Gly Leu Asp Val Cys Lys Glu Val Pro Arg Arg Arg Trp Asp 
            100                 105                 110         


Ile Asn Thr His Val Asp Pro Ser Gly Lys Ala Arg Asn Lys Gly Ala 
        115                 120                 125             


Thr Lys Trp Gly Cys Trp Leu Asp Phe Ser Gly Glu Phe Asp Pro Arg 
    130                 135                 140                 


Phe Phe Gly Ile Ser Pro Lys Glu Ala Pro Gln Met Asp Pro Ala Gln 
145                 150                 155                 160 


Arg Met Ala Leu Met Ser Thr Tyr Glu Ala Met Glu Arg Ala Gly Leu 
                165                 170                 175     


Val Pro Asp Thr 
            180 


<210>  15
<211>  177
<212>  PRT
<213>  Aspergillus niger

<400>  15

Pro Asp Lys Thr Tyr Leu Leu Ala Gly Gly Leu Gly Gly Leu Gly Arg 
1               5                   10                  15      


Thr Leu Ala Glu Trp Met Leu Gln Arg Asn Ala Lys His Leu Val Phe 
            20                  25                  30          


Leu Ser Arg Ser Gly Glu Thr Arg Ala Glu Ala Lys Ala Thr Val Ser 
        35                  40                  45              


Trp Leu Arg Ala His Gly Ile Asp Val Thr Val Tyr Lys Gly Asp Val 
    50                  55                  60                  


Ala Asn Pro Ala Asp Val Gln Ala Cys Val Gly Gly Ile Arg Asn Leu 
65                  70                  75                  80  


Gly Gly Val Phe His Ala Ala Met Val Leu Ala Asp Ala Ala Leu Glu 
                85                  90                  95      


Asn Met Thr Tyr Ala Gln Trp His Gln Cys Val Gln Pro Lys Val Val 
            100                 105                 110         


Gly Ala Phe Asn Leu His Gln Ala Thr Lys Ser Leu Pro Leu Asp Phe 
        115                 120                 125             


Phe Val Thr Phe Ser Ser Val Ser Ala Cys Phe Gly Thr Arg Ser Gln 
    130                 135                 140                 


Gly Asn Tyr Ala Ala Ala Asn Thr Tyr Leu Asp Ala Leu Met Arg Tyr 
145                 150                 155                 160 


Arg Arg Gln Ile Gly Leu Pro Ala Ala Thr Met Asn Cys Gly Arg Ile 
                165                 170                 175     


Thr 
    


<210>  16
<211>  270
<212>  PRT
<213>  Aspergillus fumigatus

<400>  16

Gln Asp Ile Asp Thr Tyr Phe Ile Pro Gly Gly Asn Arg Ala Phe Thr 
1               5                   10                  15      


Pro Gly Arg Ile Asn Tyr Tyr Phe Lys Phe Ser Gly Pro Ser Val Ser 
            20                  25                  30          


Val Asp Thr Ala Cys Ser Ser Ser Leu Ala Ala Ile His Leu Ala Cys 
        35                  40                  45              


Asn Ala Ile Trp Arg Asn Asp Cys Asp Thr Ala Ile Ser Gly Gly Val 
    50                  55                  60                  


Asn Leu Leu Thr Asn Pro Asp Asn His Ala Gly Leu Asp Arg Gly His 
65                  70                  75                  80  


Phe Leu Ser Arg Thr Gly Asn Cys Asn Thr Phe Asp Asp Gly Ala Asp 
                85                  90                  95      


Gly Tyr Cys Arg Ala Asp Gly Val Gly Thr Ile Val Leu Lys Arg Leu 
            100                 105                 110         


Glu Asp Ala Glu Ala Asp Asn Asp Pro Ile Leu Gly Val Ile Asn Ala 
        115                 120                 125             


Ala Tyr Thr Asn His Ser Ala Glu Ala Val Ser Ile Thr Arg Pro His 
    130                 135                 140                 


Val Gly Ala Gln Ala Phe Ile Phe Asn Lys Leu Leu Asn Asp Thr Asn 
145                 150                 155                 160 


Thr Asn Pro His Glu Ile Gly Tyr Val Glu Met His Gly Thr Gly Thr 
                165                 170                 175     


Gln Ala Gly Asp Ala Val Glu Met Gln Ser Val Leu Asp Val Phe Ala 
            180                 185                 190         


Pro Asp Tyr Arg Arg Gly Pro Ala Asn Ser Leu Tyr Leu Gly Ser Ala 
        195                 200                 205             


Lys Ser Asn Ile Gly His Gly Glu Ser Ala Ser Gly Val Thr Ser Leu 
    210                 215                 220                 


Val Lys Val Leu Leu Met Leu Lys Gln Asn Met Ile Pro Pro His Cys 
225                 230                 235                 240 


Gly Ile Lys Thr Lys Ile Asn His Asn Phe Pro Thr Asp Leu Ala Gln 
                245                 250                 255     


Arg Asn Val His Ile Ala Phe Lys Pro Thr Pro Trp Asn Arg 
            260                 265                 270 


<210>  17
<211>  280
<212>  PRT
<213>  Aspergillus flavus

<400>  17

Thr Ser Asp Asp Tyr Arg Glu Ile Asn Ser Gly Gln Asp Ile Asp Thr 
1               5                   10                  15      


Tyr Phe Ile Pro Gly Gly Asn Arg Ala Phe Thr Pro Gly Arg Ile Asn 
            20                  25                  30          


Tyr Tyr Phe Lys Phe Ser Gly Pro Ser Val Ser Val Asp Thr Ala Cys 
        35                  40                  45              


Ser Ser Ser Leu Ala Ala Ile His Met Ala Cys Asn Ser Ile Trp Arg 
    50                  55                  60                  


Asn Asp Cys Asp Ala Ala Ile Ala Gly Gly Val Asn Ile Leu Thr Asn 
65                  70                  75                  80  


Pro Asp Asn His Ala Gly Leu Asp Arg Gly His Phe Leu Ser Arg Thr 
                85                  90                  95      


Gly Asn Cys Asn Thr Phe Asp Asp Gly Ala Asp Gly Tyr Cys Arg Ala 
            100                 105                 110         


Asp Gly Val Gly Thr Ile Ile Leu Lys Arg Leu Glu Asp Ala Gln Ala 
        115                 120                 125             


Asp Asn Asp Pro Ile Leu Gly Val Ile Asn Gly Ala Tyr Thr Asn His 
    130                 135                 140                 


Ser Ala Glu Ala Val Ser Ile Thr Arg Pro His Val Gly Ala Gln Ala 
145                 150                 155                 160 


Phe Ile Phe Asn Lys Leu Leu Asn Asp Ala Asn Ile Asp Pro Lys Asp 
                165                 170                 175     


Val Ser Tyr Val Glu Met His Gly Thr Gly Thr Gln Ala Gly Asp Ala 
            180                 185                 190         


Val Glu Met Gln Ser Val Leu Asp Thr Phe Ala Pro Asp Tyr Arg Arg 
        195                 200                 205             


Gly Pro Gly Gln Ser Leu His Leu Gly Ser Ala Lys Ala Asn Val Gly 
    210                 215                 220                 


His Gly Glu Ser Ala Ser Gly Val Thr Ala Leu Val Lys Val Leu Leu 
225                 230                 235                 240 


Met Met Lys Lys Asn Thr Ile Pro Pro His Cys Gly Ile Lys Thr Lys 
                245                 250                 255     


Ile Asn His Asn Phe Pro Thr Asp Leu Ala Gln Arg Asn Val His Ile 
            260                 265                 270         


Ala Phe Gln Pro Thr Pro Trp Asn 
        275                 280 


<210>  18
<211>  300
<212>  PRT
<213>  Aspergillus niger

<400>  18

Asn Arg Ala Phe Thr Pro Gly Arg Ile Asn Tyr Tyr Phe Lys Phe Ser 
1               5                   10                  15      


Gly Pro Ser Val Ser Val Asp Thr Ala Cys Ser Ser Ser Leu Ala Ala 
            20                  25                  30          


Ile His Met Ala Cys Asn Ser Ile Trp Arg Asn Asp Cys Asp Ala Ala 
        35                  40                  45              


Ile Thr Gly Gly Val Asn Ile Leu Thr Ser Pro Asp Asn His Ala Gly 
    50                  55                  60                  


Leu Asp Arg Gly His Phe Leu Ser Thr Thr Gly Asn Cys Asn Thr Phe 
65                  70                  75                  80  


Asp Asp Gly Ala Asp Gly Tyr Cys Arg Ala Asp Gly Val Gly Ser Ile 
                85                  90                  95      


Val Leu Lys Arg Leu Glu Asp Ala Glu Ala Asp Asn Asp Pro Ile Leu 
            100                 105                 110         


Ala Val Ile Asn Gly Ala Tyr Thr Asn His Ser Ala Glu Ala Val Ser 
        115                 120                 125             


Ile Thr Arg Pro His Val Gly Ala Gln Ala Phe Ile Phe Asn Lys Leu 
    130                 135                 140                 


Leu Asn Asp Ala Asn Ile Asp Pro Lys Asp Val Ser Tyr Val Glu Met 
145                 150                 155                 160 


His Gly Thr Gly Thr Gln Ala Gly Asp Ala Val Glu Met Gln Ser Val 
                165                 170                 175     


Leu Asp Val Phe Ala Pro Asp Tyr Arg Arg Gly Pro Gly Gln Ser Leu 
            180                 185                 190         


His Ile Gly Ser Ala Lys Ala Asn Ile Gly His Gly Glu Ser Ala Ser 
        195                 200                 205             


Gly Val Thr Ala Leu Val Lys Val Leu Leu Met Met Arg Glu Asn Met 
    210                 215                 220                 


Ile Pro Pro His Cys Gly Ile Lys Thr Lys Ile Asn Ser Asn Phe Pro 
225                 230                 235                 240 


Thr Asp Leu Ala Lys Arg Asn Val His Ile Ala Phe Gln Pro Thr Pro 
                245                 250                 255     


Trp Asn Arg Pro Ala Ser Gly Lys Arg Arg Thr Phe Val Asn Asn Phe 
            260                 265                 270         


Ser Ala Ala Gly Gly Asn Thr Ala Leu Leu Leu Glu Asp Ala Pro Ile 
        275                 280                 285             


Pro Glu Arg Gln Gly Gln Asp Pro Arg Ser Phe His 
    290                 295                 300 


